Composite

Part:BBa_K4147003:Design

Designed by: César Ibrahym Rodríguez Fernández, Ana Belem García González   Group: iGEM22_Tec-Chihuahua   (2022-09-30)


PcOSM Construct with LacI regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When sending the part to synthetize, be sure to add some extra bases at the ends of the prefix and suffix, otherwise, the restriction enzymes might not be able to digest, making it difficult to later ligate in a vector.


Source

GenBank: EU271754.1 https://www.ncbi.nlm.nih.gov/nuccore/EU271754.1